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SOX2-RT-PCR real time
Quantification assay of stemness markers SOX2 cod. BM-010
Principle of the test: Quantitative analysis of SOX2 mRNA expression
Technology: Relative Quantitative Real Time PCR
Gene Target: SOX2
Specimen: cDNA
Results within: ΔΔCt method
Reporting Units: Arbitrary Units (AU)
Number of tests: 25 tests BM-010
Kit storage: -20°C
Necessary equipment: Thermocycler, 7500 Real Time PCR System
Status: Ready to use
SOX2-RT-PCR real time cod. BM-010
Quantification assay of stemness markers SOX2
· SOX2-RT-PCR real time Quantification complete kit 25 tests BM-010
Sox2 is a member of the Sox family of transcription factors. Sox2 is classified as a member of SoxB1 group, which also includes Sox1 and Sox3. Although Sox1, Sox2 and Sox3 share more than 80% sequence similarity and are functionally redundant, Sox2 can exert distinct functions in a biologically context-dependent manner and is indispensable for embryonic development. In human and mouse ESCs, Oct4, Sox2 and Nanog form a core transcriptional regulatory circuitry in pluripotent stem cells to maintain their self-renewal. Oct4 and Sox2 co-occupy a large number of enhancers/promoters and regulate the expression levels of their target genes21.
We have established a novel quantitative analysis of SOX2 mRNA expression. This method is based on real-time PCR. The expression levels of the mRNAs were determined from the threshold cycle (Ct), and the relative expression levels were calculated using the 2-ΔΔCt method. For mRNA quantification, the Ct values were normalized to the expression of the GAPDH mRNA level. Results are expressed in corresponding arbitrary units (AU) User friendly and complete, the SOX2-RT-PCR real time Quantification kit is suitable for any laboratory.
Reference
1. Zhang S, Cui W. Sox2, a key factor in the regulation of pluripotency and neural differentiation. World J Stem Cells. 2014 Jul 26;6(3):305-11. doi: 10.4252/wjsc.v6.i3.305