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OSTEO-RT-PCR real time

Quantification assay for osteogenic differentiation of MSCs         cod. BM-017

Principle of the test: Quantitative analysis of COL11A1 mRNA expression

Technology: Relative Quantitative PCR

Gene Target: COL11A1

Specimen: cDNA

Results: ΔΔCt method

Reporting Units: arbitrary units (AU)

Number of tests: 25 tests BM-017

Kit storage: -20°C

Necessary equipment: 7500 Real Time PCR System

Status: Ready to use

OSTEO-RT-PCR real time  cod. BM-017

Quantification assay for osteogenic differentiation of MSCs

·      Osteo-RT-PCR real time Quantification complete kit 25 tests BM-017

The precise predictions of the differentiation direction and potential of MSCs are an important key to the success of regenerative medicine. MSCs can differentiate into various cell types, including osteoblasts, chondrocytes, or adipocytes; therefore, they are promising as regenerative medicine. We have developed a gene-specific differentiation assay to analyze when a mesenchymal cell has switched its fate to an osteogenic lineage. We have established a novel quantitative analysis of COL11A1 mRNA expression. COL11A1 is abundantly secreted by MSCs and can be further up-regulated during the osteogenic differentiation of these cells. This method is based on real-time PCR. The expression levels of the mRNAs were determined from the threshold cycle (Ct), and the relative expression levels were calculated using the 2-ΔΔCt method. For mRNA quantification, the Ct values were normalized to the expression of the GAPDH mRNA level. Results are expressed in corresponding arbitrary units (AU) User friendly and complete, the Osteo-RT-PCR real time Quantification kit is suitable for any laboratory.

Reference

1.      Rickard DJ, Sullivan TA, Shenker BJ, et al. Induction of rapid osteoblast differentiation in rat bone marrow stromal cell cultures by dexamethasone and BMP-2. Dev Biol. 1994;161:218–228