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IFNsig. type II One Step cod. BM-024 - Multiplex One Step RT-PCR Real Time Cod. BM-024
Principle of the test : Quantitative analysis of Interferon Stimulated Genes ISG
Technology : Multiplex One Step RT- PCR Real Time
Gene Target : IFNγ, IDO1, CXCL9, CXCL10, STAT1, HLA DRA
Internal Control: GAPDH
Specimen : RNA
Results : ΔΔCt method
Reporting Units : Arbitrary Units (AU)
Number of tests : 25 tests BM-023
Kit storage : -20°C
Necessary equipment : 7500 Real Time PCR System or CFX96 DetectionSystem
IFNsig. Type II One Step cod. BM-024
Multiplex One Step RT-PCR Real Time Six Targets Two tubes
Type II interferon (IFN), known as IFN-γ, while sharing a similar nomenclature to type I IFN, binds to a different receptor and has independent effects. A component of the innate immune response, produced mainly by natural killer cells during infections (1). IFN-γ is a key cytokine also produced by activated T cells, as well as by natural killer (NK) and NK T cells, in the tumour microenvironment and plays an important role in the coordination of this process. In addition, IFN-γ can stimulate the expression of other key immunosuppressive molecules such as IDO1, CXCL9 and CXCL10. The expression of these molecules represents the IFN-γ signature or IFN type II signature (1). Recently, Imgenberg-Kreuz and colleagues presented a comprehensive transcriptome profile in B-cells from patients with primary autoantibody Sjogren's syndrome (pSS) that identifies large differences in the expression patterns of patients compared to controls, including targets of type I and type II interferon signatures (2).
The IFNsig.Type II Multiplex One Step kit is a real-time PCR assay able to reverse-transcribe and amplify RNA in a single step. Thanks to the multiplex version, the RNA is tested with two different mixes that will return the data of the six genes stimulated by Interferon α and the housekeeping gene. The relative quantification of the gene expression of these targets is performed with the ΔΔCt method (5) and the fold change obtained is expressed in arbitrary units. Easy to use and complete, the IFNsig.Type II One Step kit is an optimal solution for all molecular diagnostics laboratories.
Reference:
1. Lee AJ, Ashkar AA. The Dual Nature of Type I and Type II Interferons. Front Immunol. 2018 Sep 11;9:2061. doi: 10.3389/fimmu.2018.02061
2. Imgenberg-Kreuz J, Sandling JK, Björk A, Nordlund J, Kvarnström M, Eloranta ML, Rönnblom L, Wahren-Herlenius M, Syvänen AC, Nordmark G. Transcription profiling of peripheral B cells in antibody-positive primary Sjögren's syndrome reveals upregulated expression of CX3CR1 and a type I and type II interferon signature. Scand J Immunol. 2018 May;87(5):e12662. doi: 10.1111/sji.12662.
3. Min Ae Lee-Kirsch. The Type I Interferonopathies. Annual Review of Medicine 2017;68:297-315
4. PIn A, Monasta L, Taddio A, Piscianz E, Tommasini A, Tesser A. An Easy and Reliable Strategy for Making Type I Interferon Signature Analysis Comparable among Research Centers. Diagnostics (Basel). 2019;9(3):113. doi: 10.3390/diagnostics9030113.
5. Kenneth J. Livak and Thomas D. Schmittgen. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2-[Delta][Delta]CT Method. Methods, 25(4):402 – 408, 2001